| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090194 |
| Chromosome: | chromosome 1 |
| Location: | 4177657 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027600 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCCTGGGTCTGGGTCTACTAGGGCTGA |
| Internal bar code: | ACGTAAGGGCCCTAATATCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 91.8634 |
| LEAP-Seq n confirming: | 38127 |
| LEAP-Seq n nonconfirming: | 3377 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCTGTAATGAAGGCTGG |
| Suggested primer 2: | TAGCAGTAGCAGTAGCGGCA |