| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090228 |
| Chromosome: | chromosome 12 |
| Location: | 2515535 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506050 | (1 of 1) K14408 - cleavage stimulation factor subunit 3 (CSTF3, RNA14) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGGCAGGTAGCAACCGCAATTACGCAA |
| Internal bar code: | AAGTGCATTGAATCCGAGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 98.0308 |
| LEAP-Seq n confirming: | 3186 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACGGCATGTAAGACGGT |
| Suggested primer 2: | GTTGCTGCCCTCTTGCTTAC |