| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.090250 |
| Chromosome: | chromosome 1 |
| Location: | 2711358 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016100 | (1 of 7) IPR011048 - Cytochrome cd1-nitrite reductase-like, haem d1 domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTTCCGTAGTACGTTTCATCTGGCCTT |
| Internal bar code: | ACAAGGCAGTTGGCTTGAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 783 |
| LEAP-Seq percent confirming: | 99.439 |
| LEAP-Seq n confirming: | 8686 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTAGTGGCCTGGTTCTT |
| Suggested primer 2: | CGCAAGTCTTTGACCTCTCC |