| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090254 |
| Chromosome: | chromosome 17 |
| Location: | 762440 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g701450 | (1 of 12) PTHR13078:SF22 - MAOC-LIKE DEHYDRATASE DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTAGACCTGGATGTCCTAGCCTCAGCC |
| Internal bar code: | CCTAGGGTCGTCGATTTCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 338 |
| LEAP-Seq percent confirming: | 99.2499 |
| LEAP-Seq n confirming: | 2911 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTAAAGGCCAGTTCC |
| Suggested primer 2: | TGTTTTTGGTTGCATCGGTA |