| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090263 |
| Chromosome: | chromosome 13 |
| Location: | 1215648 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570250 | CKIN1L,SNRK1B,CKIN2 | (1 of 1) PF07714//PF14531 - Protein tyrosine kinase (Pkinase_Tyr) // Kinase-like (Kinase-like); snRK1 family in Chlamydomonas, subgroup 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTATGACTACCAGTAAGAATCCGTTTCA |
| Internal bar code: | TGTTGTGCGCAACGCATCTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 99.6654 |
| LEAP-Seq n confirming: | 3872 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTCTGTGTGGCCAATTT |
| Suggested primer 2: | GGCTACCGGTATGTTGTGCT |