| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.090269 |
| Chromosome: | chromosome_2 |
| Location: | 458681 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g076466 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TACAAGCTCGTCGTTATCTCGCGAATCCAG |
| Internal bar code: | CCAGTAGACCCCGACGCCCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 98.1854 |
| LEAP-Seq n confirming: | 3517 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTAGACCAAACACATGG |
| Suggested primer 2: | GTTGAGGTGAAGCTGAAGGC |