| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.090282 |
| Chromosome: | chromosome 12 |
| Location: | 1944019 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g510550 | (1 of 2) PTHR12197//PTHR12197:SF145 - SET AND MYND DOMAIN CONTAINING // SET DOMAIN-CONTAINING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGATGAAGTGCGGGTGCTCGGCCATGGA |
| Internal bar code: | GGTAACGGAGGGTTCGCATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 361 |
| LEAP-Seq percent confirming: | 99.3584 |
| LEAP-Seq n confirming: | 3562 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACGTACCGAGATTCAAG |
| Suggested primer 2: | GGTAAACGCTTCCGCATTAG |