Insertion junction: LMJ.RY0402.090285_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.090285
Chromosome:chromosome 9
Location:937334
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g401022 SNE3, UXE,UXE1 UDP-xylose-4-epimerase; (1 of 1) K12448 - UDP-arabinose 4-epimerase (UXE, uxe) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACACACATATACACACACATACATTTGGG
Internal bar code:GTGGATGATCGTGTCGCCCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:116
LEAP-Seq percent confirming:99.6127
LEAP-Seq n confirming:1286
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCTAGGAATTGCAGGAGTGC
Suggested primer 2:TCCTGCGCTACTTCAATGTG