| Insertion cassette: | CIB1 | 
| Side of cassette: | 5' | 
| Strand: | - | 
| Strain: | LMJ.RY0402.090305 | 
| Chromosome: | chromosome 2 | 
| Location: | 7868855 | 
| Confidence (%): | 58 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre02.g141700 | FAP315 | (1 of 2) PTHR12932//PTHR12932:SF9 - P25 ALPHA-RELATED // TPPP FAMILY PROTEIN CG45057; EF-Hand Containing Flagellar Associated Protein 315 | intron | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCCCACGTCCACCACTCCACCCTCCAC | 
| Internal bar code: | ATATATCGATATATACGTGTC | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 7 | 
| LEAP-Seq percent confirming: | 100.0 | 
| LEAP-Seq n confirming: | 39 | 
| LEAP-Seq n nonconfirming: | 0 | 
| LEAP-Seq n unique pos: | 2 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCACAAATCACCAAAGG | 
| Suggested primer 2: | AAGGTGGTAGTGTCAACCGC |