Insertion junction: LMJ.RY0402.090305_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.090305
Chromosome:chromosome 2
Location:7868855
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g141700 FAP315 (1 of 2) PTHR12932//PTHR12932:SF9 - P25 ALPHA-RELATED // TPPP FAMILY PROTEIN CG45057; EF-Hand Containing Flagellar Associated Protein 315 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTCCCACGTCCACCACTCCACCCTCCAC
Internal bar code:ATATATCGATATATACGTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:7
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCACAAATCACCAAAGG
Suggested primer 2:AAGGTGGTAGTGTCAACCGC