Insertion junction: LMJ.RY0402.090311_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.090311
Chromosome:chromosome 10
Location:872508
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g423850 SRR26 Scavenger receptor cysteine rich (SRCR) protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCAACTTATCGGCCGCAGGCCGTAGCC
Internal bar code:GGCACTTTAATGTCGGACGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:561
LEAP-Seq percent confirming:81.1494
LEAP-Seq n confirming:353
LEAP-Seq n nonconfirming:82
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTGTCTTTTGATTAGGC
Suggested primer 2:GCTTCTCGGTGCCAGACTAC