Insertion junction: LMJ.RY0402.090311_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.090311
Chromosome:chromosome 10
Location:872564
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g423850 SRR26 Scavenger receptor cysteine rich (SRCR) protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCCTCTGCGATTCCATGATATCTGTACA
Internal bar code:AATATATTTTGTGTACCTTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0979432
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:2040
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTGTCTTTTGATTAGGC
Suggested primer 2:GCGTTTGGCTATTGGTTTGT