Insertion junction: LMJ.RY0402.090317_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.090317
Chromosome:chromosome 7
Location:183734
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTTTCGCCTACTGCGCGACCAGCGCCTT
Internal bar code:GCCCGTGACCGCGGGACCCAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:491
LEAP-Seq percent confirming:97.8774
LEAP-Seq n confirming:415
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGTGCACCTTGCTCGATA
Suggested primer 2:GACGCTATCTCTGCCTCCTG