| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.090326 |
| Chromosome: | chromosome 13 |
| Location: | 2881425 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g583450 | EZY17 | Early zygote expressed protein; (1 of 6) PTHR15907:SF21 - DUF614 FAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGATACTACAGCGCTAATGCGCGTAAAAG |
| Internal bar code: | TACGATAACGTATCCGACCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1071 |
| LEAP-Seq percent confirming: | 99.437 |
| LEAP-Seq n confirming: | 2826 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTCCATGCTCGAGTACC |
| Suggested primer 2: | CTGAAGGGTTTGAGACGAGC |