| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090335 |
| Chromosome: | chromosome 2 |
| Location: | 5377809 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107256 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGCTGCACTCGCGATTAGCAGGCAGA |
| Internal bar code: | GGGATACGACCGTACTTATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 934 |
| LEAP-Seq percent confirming: | 99.57 |
| LEAP-Seq n confirming: | 7410 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCTAGGAAATGGATCAG |
| Suggested primer 2: | CTCTGAAAGAGGCTTGTGGG |