Insertion junction: LMJ.RY0402.090337_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.090337
Chromosome:chromosome 9
Location:3805393
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g391615 (1 of 80) IPR003882 - Pistil-specific extensin-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTCGAAGTAGAAGAGGCCCATGGTCGTC
Internal bar code:CAGCCGCTCGGTGCGACCGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:658
LEAP-Seq percent confirming:99.3871
LEAP-Seq n confirming:5513
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGTTCGAGAGGGTACATA
Suggested primer 2:CCGTTTCAGTCTGCTGTCAA