Insertion junction: LMJ.RY0402.090385_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.090385
Chromosome:chromosome 2
Location:283800
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g075050 PTA1 (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACCGCATCGGGCGCAAATGGGGCTCTGT
Internal bar code:CCGCAGTCGCGATTGCGATTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:551
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:130
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGATGTTTGGAAAAGCGGTA
Suggested primer 2:CTCGCCATCTGCTTGACATA