| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090387 |
| Chromosome: | chromosome 12 |
| Location: | 2628881 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504650 | HTR15,HTR28 | Histone H3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGATGCGGGCCGCGGGCCGTTATGCAG |
| Internal bar code: | GAGGTATTGGTTCAAATTCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1034 |
| LEAP-Seq percent confirming: | 99.4723 |
| LEAP-Seq n confirming: | 5090 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGAGGACACCAACCTGT |
| Suggested primer 2: | CTGCACTCCCATGTCTCTGA |