| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090390 |
| Chromosome: | chromosome 1 |
| Location: | 7480913 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053750 | LPL3 | (1 of 1) 2.7.7.63 - Lipoate--protein ligase / LPL; Putative lipoate A/B protein ligase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAACAATGTTGCAGTGTACGCGCTGCG |
| Internal bar code: | AAGGAGGGCCGGGGTGGCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 98.9387 |
| LEAP-Seq n confirming: | 1678 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGACAACTGCTTTCACG |
| Suggested primer 2: | ATGAGAGAAGCGCGAATTGT |