| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.090415 |
| Chromosome: | chromosome 3 |
| Location: | 6342883 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193700 | (1 of 1) PTHR10992//PTHR10992:SF749 - ALPHA/BETA HYDROLASE FOLD-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTGTGTGTAGCTCGAGATTCTAGTACTT |
| Internal bar code: | CGGACATTCCTGTAGTCTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 98.2355 |
| LEAP-Seq n confirming: | 3062 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCAATGTACTTTCGTGTG |
| Suggested primer 2: | GAGCTGTCTTCACGAAAGGG |