Insertion junction: LMJ.RY0402.090437_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.090437
Chromosome:chromosome 10
Location:4817135
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g454000 (1 of 2) IPR000157//IPR016024 - Toll/interleukin-1 receptor homology (TIR) domain // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACCCACGCACCCCCGCGCCCACCTGGTC
Internal bar code:TAACACGGAAGGGCTAGGACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:355
LEAP-Seq percent confirming:98.1818
LEAP-Seq n confirming:378
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTGAGTGAGGGGAGGT
Suggested primer 2:AGGAGGAGAGGAGGGGTGTA