Insertion junction: LMJ.RY0402.090468_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.090468
Chromosome:chromosome 14
Location:2651789
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g626400 CYP33,CYP743C1 (1 of 3) 1.14.14.1//5.3.99.5 - Unspecific monooxygenase / Xenobiotic monooxygenase // Thromboxane-A synthase / Thromboxane synthetase; Cytochrome P450, CYP197 superfamily 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCACGCGCCACCCCTCTGCCGTAGCCGCG
Internal bar code:TTTTCTTTGAGCGGTTGGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:988
LEAP-Seq percent confirming:99.1476
LEAP-Seq n confirming:20937
LEAP-Seq n nonconfirming:180
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:CCATGGGGTTAGAGGAGTGA
Suggested primer 2:GGTTGATAGGAGGCGTGTGT