| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.090469 |
| Chromosome: | chromosome 6 |
| Location: | 1014440 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256000 | (1 of 1) PF15996 - Arginine/serine-rich protein PNISR (PNISR) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGTTGTGGCATGTGCTTCGTGCTGCAT |
| Internal bar code: | GGTCATGCGCGATTACTGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 99.1327 |
| LEAP-Seq n confirming: | 3429 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACGGCAAGAAGGAGAAGA |
| Suggested primer 2: | CACACATGAACGGGAGTGAC |