| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.090492 |
| Chromosome: | chromosome_4 |
| Location: | 2002136 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g218800 | THB3 | Truncated hemoglobin | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACCGCTCATGTACGGTACGATACGGCACG |
| Internal bar code: | GGTTCGATAGCTGGTGTCACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 419 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 127 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCATGCCCAATGATATAG |
| Suggested primer 2: | ACTTGCATCTGGGTTTGTCC |