Insertion junction: LMJ.RY0402.090527_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.090527
Chromosome:chromosome 3
Location:8922578
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g208497 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTAATCGTCACAGCTCACAGCTACTTTA
Internal bar code:AGGTCGGGTCCAGAGACGCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:647
LEAP-Seq percent confirming:97.4734
LEAP-Seq n confirming:733
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CACACTGTGCTGAGAGCCAT
Suggested primer 2:TGAGACGACCTTGCTGTCAC