| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.090598 |
| Chromosome: | chromosome 7 |
| Location: | 3294288 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334450 | EFH4 | EF-hand domain protein; (1 of 1) IPR007044 - Cyclodeaminase/cyclohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAAACGAGCCATGCACGAAAGTGTAAAC |
| Internal bar code: | GTTAGGCGAGCATTAGCAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 99.6707 |
| LEAP-Seq n confirming: | 2119 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGACCGATAACGAGGTG |
| Suggested primer 2: | GGGTCGATCTGGTACATGCT |