| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090604 |
| Chromosome: | chromosome 13 |
| Location: | 2976331 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584100 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGAGAAGATAAACAGGCCACGGGCATGT |
| Internal bar code: | GACTAGCGCGACATCAGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 679 |
| LEAP-Seq percent confirming: | 98.2225 |
| LEAP-Seq n confirming: | 2763 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGCTTGAAACAGTCGGG |
| Suggested primer 2: | CGGGTGACCAACAGTTTTCT |