| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.090610 |
| Chromosome: | chromosome 3 |
| Location: | 9174023 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g212753 | (1 of 1) PTHR21496//PTHR21496:SF6 - FERREDOXIN-RELATED // 3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE-LIKE PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCCGGCCTTGGCGCGGGCCTCCTCGTT |
| Internal bar code: | TCCACGGATAGCGAGCGTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 525 |
| LEAP-Seq percent confirming: | 99.1531 |
| LEAP-Seq n confirming: | 1522 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTATTGCTCACAGGCTCG |
| Suggested primer 2: | GAGCTGTCATGGGTGTCAGA |