| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.090615 |
| Chromosome: | chromosome 3 |
| Location: | 4162061 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173700 | KIL14 | Putative kinesin-like motor protein; (1 of 1) K10396 - kinesin family member 5 (KIF5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCGGGCATGCCTCAACCGTACCAGATG |
| Internal bar code: | TGCGTACCGCGGGTTGAACAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCACGCTAGGAGGACAC |
| Suggested primer 2: | CACACACACACACACACCGT |