| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090623 |
| Chromosome: | chromosome 11 |
| Location: | 256617 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467557 | (1 of 2) PTHR31389:SF4 - PROTEIN F32B4.1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTACGAGATGCGCAACCTGCGCACCTAC |
| Internal bar code: | GGCACAAGTCGTGCCTTGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 131 |
| LEAP-Seq percent confirming: | 99.6815 |
| LEAP-Seq n confirming: | 626 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGGTGGCTATGGTTGTA |
| Suggested primer 2: | TCTAGAGCTGCTCACACGGA |