| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090624 |
| Chromosome: | chromosome 16 |
| Location: | 5166834 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683400 | (1 of 10) PTHR10174 - RETINALDEHYDE BINDING PROTEIN-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTGTGAGGAACTAACCGACGGCCCGACT |
| Internal bar code: | TTGGACACGGTTGTGGCCCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 99.3805 |
| LEAP-Seq n confirming: | 3529 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCGGAGTAGAGGCAGTGA |
| Suggested primer 2: | GGCCTTCACAACTCACCAAT |