| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090628 |
| Chromosome: | chromosome 2 |
| Location: | 4742629 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102300 | (1 of 1) PTHR31013 - THAUMATIN FAMILY PROTEIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCACGCCGCCGATCCAGACGCCGCCGCT |
| Internal bar code: | CTCGCGGGCTGAGGTTAGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 598 |
| LEAP-Seq percent confirming: | 99.3939 |
| LEAP-Seq n confirming: | 164 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTTGTTGCTTGCTGAAG |
| Suggested primer 2: | GTGGAGAGTGTCGAGCATCA |