| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090633 |
| Chromosome: | chromosome 3 |
| Location: | 2393585 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158950 | (1 of 1) K12833 - pre-mRNA branch site protein p14 (SF3B14) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGGACGTGGCAGCAATGGACTAGCTGT |
| Internal bar code: | TACCAGCCGGTCCAGTATCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 78.3399 |
| LEAP-Seq n confirming: | 991 |
| LEAP-Seq n nonconfirming: | 274 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGCAGGGCTCTATTCGT |
| Suggested primer 2: | GAAGTGGTGTCGTGGTGATG |