Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.090651 |
Chromosome: | chromosome_1 |
Location: | 915603 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g004900 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TATGAATTGGCATGTTATACTGAAGCTGTT |
Internal bar code: | AGTTTGTACTATGTTTTGTAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 760 |
LEAP-Seq percent confirming: | 99.439 |
LEAP-Seq n confirming: | 4609 |
LEAP-Seq n nonconfirming: | 26 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGTGGGACAAGCTCGTAT |
Suggested primer 2: | GCCTCTCCACCTCTGTCAAG |