| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.090651 |
| Chromosome: | chromosome_1 |
| Location: | 915615 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g004900 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGACTTCTTTGGGGGCTGCCCGCTGCGCTT |
| Internal bar code: | ACTCGGTGTCGGTGTGACATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 98.522 |
| LEAP-Seq n confirming: | 4866 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTGGGACAAGCTCGTAT |
| Suggested primer 2: | GCCTCTCCACCTCTGTCAAG |