| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090689 |
| Chromosome: | chromosome 17 |
| Location: | 2974293 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720183 | (1 of 1) K08998 - hypothetical protein (K08998) | 5'UTR|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGACGTTTAAGAGGTGGAGCTTTAGGCC |
| Internal bar code: | ACCCGTGTATTAATCCCCAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 95.6425 |
| LEAP-Seq n confirming: | 2568 |
| LEAP-Seq n nonconfirming: | 117 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTAAGGTGGCATGTAAT |
| Suggested primer 2: | GTCCCTTCTCCCTCTGATCC |