| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090691 |
| Chromosome: | chromosome 16 |
| Location: | 844859 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647800 | (1 of 1) IPR000104//IPR001932//IPR015655 - Antifreeze protein, type I // PPM-type phosphatase domain // Protein phosphatase 2C | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAGTGGGCGCCCATGTCGTAGGCCAGG |
| Internal bar code: | GGTCGCAGGGCCCGAAATCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 80.9214 |
| LEAP-Seq n confirming: | 7711 |
| LEAP-Seq n nonconfirming: | 1818 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTATTAAACGCGTGCCTA |
| Suggested primer 2: | GTTGTGCTTGATGGTGGTTG |