| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090697 |
| Chromosome: | chromosome 1 |
| Location: | 6227396 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCAGGCTGAGGGGCTGTGCAAGGAGGA |
| Internal bar code: | GGCCTATGGGGTTCTTTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 99.7148 |
| LEAP-Seq n confirming: | 9441 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCGCCCTCTACCTGTTG |
| Suggested primer 2: | GATATGCTTCGGCACTCCAT |