Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.090706 |
Chromosome: | chromosome 11 |
Location: | 3285859 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g480100 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGTCGGTACCAAATGACAGTTCACGGA |
Internal bar code: | AGACAGCTAGCACGCCCGCGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 707 |
LEAP-Seq percent confirming: | 93.1439 |
LEAP-Seq n confirming: | 3967 |
LEAP-Seq n nonconfirming: | 292 |
LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCTTTCAATCGCAATTTGT |
Suggested primer 2: | TACGGTAACTGGAGTTGGGC |