| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090706 |
| Chromosome: | chromosome 11 |
| Location: | 3285863 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCGTCGTGGAACTGACACCTGCCGCACA |
| Internal bar code: | GGCCTCGACGGTACCCTAAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1405 |
| LEAP-Seq percent confirming: | 99.7012 |
| LEAP-Seq n confirming: | 9344 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTTCAATCGCAATTTGT |
| Suggested primer 2: | TACGGTAACTGGAGTTGGGC |