| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.090717 |
| Chromosome: | chromosome_3 |
| Location: | 8789356 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g205697 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCTGGGCATGCCCGGCACGCCCGGCATGA |
| Internal bar code: | GCTCCAGTTTCCGTGGCGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 90.6229 |
| LEAP-Seq n confirming: | 5354 |
| LEAP-Seq n nonconfirming: | 554 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCCATCATGAACATCCC |
| Suggested primer 2: | GTGTTGGTGTTGGAATGCAG |