| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090738 |
| Chromosome: | scaffold 19 |
| Location: | 37305 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750397 | (1 of 1) 3.2.1.17 - Lysozyme / Muramidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTCAACAAACACGTGGGGCATGGAGTGG |
| Internal bar code: | AAGCACGTAGCTCACAGGGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 568 |
| LEAP-Seq percent confirming: | 98.0196 |
| LEAP-Seq n confirming: | 47120 |
| LEAP-Seq n nonconfirming: | 952 |
| LEAP-Seq n unique pos: | 140 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTATGAAGTGTCGACGGGG |
| Suggested primer 2: | GGAGCTGGAGGTTGTAGCAC |