| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.090758 |
| Chromosome: | chromosome 15 |
| Location: | 864757 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639503 | GEX173 | (1 of 7) PTHR23257//PTHR23257:SF474 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGGGTCTGGTCTGGTGTTGTTGCCTG |
| Internal bar code: | GACTGACGTGCGTGTCCGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 149 |
| LEAP-Seq percent confirming: | 64.5276 |
| LEAP-Seq n confirming: | 1086 |
| LEAP-Seq n nonconfirming: | 597 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTTTGTGGACTGGGGAAA |
| Suggested primer 2: | ACGTGGTGTTTCGTGTGTGT |