| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.090790 |
| Chromosome: | chromosome 12 |
| Location: | 6930795 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560550 | MTF1 | (1 of 2) 2.1.2.9 - Methionyl-tRNA formyltransferase / transformylase; Methionyl-tRNA formyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCAGGCTCCCATCGCCTCTCCCGCATT |
| Internal bar code: | GCGAGACTGGACTATCAGTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 686 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 186 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCGATGCTGGACTTTGCT |
| Suggested primer 2: | GCGTAGCACTCTTTGGCTTC |