| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.090811 |
| Chromosome: | chromosome 16 |
| Location: | 6496653 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673000 | (1 of 1) PTHR30028 - UPF0014 INNER MEMBRANE PROTEIN YBBM-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGGGAATCGTGCACCCGTGCACGTGCT |
| Internal bar code: | AACTTTATCCACGCGGGAATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 851 |
| LEAP-Seq percent confirming: | 99.6541 |
| LEAP-Seq n confirming: | 6915 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTTGCGAAGTGCGTATC |
| Suggested primer 2: | GCGACTATCGGATGTACCGT |