| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090815 |
| Chromosome: | chromosome 6 |
| Location: | 6153136 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g290250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTACTGACGACCACTCCCTGCCCTCTT |
| Internal bar code: | GTAGGATCGCTCACACACCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 87.6171 |
| LEAP-Seq n confirming: | 8512 |
| LEAP-Seq n nonconfirming: | 1203 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGTTCGAGAAGGTGGCTC |
| Suggested primer 2: | CATAATCCGGCTCAGCAAGT |