| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.090822 |
| Chromosome: | chromosome 10 |
| Location: | 320786 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420200 | PHC55 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACTACCGCAATGCCCAGGGCATGCAGAA |
| Internal bar code: | GCTGCCTTAGTGGTCATACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 99.3772 |
| LEAP-Seq n confirming: | 2234 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAACCCTGCAGCTAGGAC |
| Suggested primer 2: | CCTCGTCCTTCTACGTCTCG |