| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090839 |
| Chromosome: | chromosome 5 |
| Location: | 3152017 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATTTTGTGCTGCTGTCATTGGCTACA |
| Internal bar code: | GCTTGTCATACGGCCATTTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 947 |
| LEAP-Seq percent confirming: | 99.3086 |
| LEAP-Seq n confirming: | 1580 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTATCGCGCTTTCACTTG |
| Suggested primer 2: | GTCACCAGCTAGCCCACAG |