| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090856 |
| Chromosome: | scaffold 19 |
| Location: | 193382 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g751197 | (1 of 1) K14794 - ribosomal RNA-processing protein 12 (RRP12) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCCATTTGATTTCATAGTTTTAGTGGG |
| Internal bar code: | ATGCGTTCATCTCCTAGACAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 226 |
| LEAP-Seq percent confirming: | 99.6169 |
| LEAP-Seq n confirming: | 260 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGTTGTCGGTTGAGTCT |
| Suggested primer 2: | CTCAAGCAGGTGGGAGAGAG |