Insertion junction: LMJ.RY0402.090870_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.090870
Chromosome:chromosome 6
Location:1918442
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g263500 (1 of 1) PF01951 - Archease protein family (MTH1598/TM1083) (Archease) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGTCAGCTACCACGGGCGGGTAACCACC
Internal bar code:TTACGCCTAACCGACGTTTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:656
LEAP-Seq percent confirming:99.5487
LEAP-Seq n confirming:8382
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AACACAGAGCTGTTCCTGGC
Suggested primer 2:TGTCCGTCACCCACAAGTAA