| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090882 |
| Chromosome: | chromosome 12 |
| Location: | 514134 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493150 | LTO1,CPLD41,VKE1 | Vitamin K epoxide reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCGGGTGTGGTCCGGACCGAAAGGCCA |
| Internal bar code: | CGGGGATGGATCTCGATTGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 86 |
| LEAP-Seq percent confirming: | 72.1254 |
| LEAP-Seq n confirming: | 414 |
| LEAP-Seq n nonconfirming: | 160 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCCTGATTCTGAACGTG |
| Suggested primer 2: | TTCCTGGAGTACAAGGTGGG |